Strain Data

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Strain number		NIES-642
Phylum		Chlorophyta
Class		Trebouxiophyceae
Scientific name		Chlorella vulgaris Beijerinck var. vulgaris
Synonym
Former name
Common name		Green alga
Locality (Date of collection)		Miyata River, Ibaraki, Japan (1987-02-25)
Latitude / Longitude
Habitat (Isolation source)		Freshwater (River water)
History		< Kasai, Fumie
Isolator (Date of isolation)		Kasai, Fumie (1987-03-23)
Identified by		Kasai, Fumie; Confirmed by DNA sequencing at NIES
State of strain		Subculture / Cryopreservation; Unialgal; Clonal; Axenic[2018 May]
Culture condition (Preculture condition)		Medium: C Temperature: 20 C Light intensity: 8-15 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 6 M
Gene information		18S rRNA ( AB488577 )
Cell size (min - max)		4 - 6 μm
Organization		Unicellular
Characteristics
Other strain no.		Other strain no. : 1st-2-17
Remarks		Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-2170	Chlorella vulgaris	Green alga		Starch production (Hirokawa et al. 1982) ; Genome decoded strain (Wakasugi et al. 1997)
NIES-1269	Chlorella vulgaris	Green alga	Marine (Tidal flat water)
NIES-641	Chlorella vulgaris	Green alga	Freshwater (River water)
NIES-686	Chlorella vulgaris	Green alga		Authentic strain
NIES-227	Chlorella vulgaris	Green alga

Related strain information (Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-2169	Chlorella sorokiniana	Green alga	Freshwater	Starch production (Takeshita et al. 2014)
NIES-2173	Chlorella sorokiniana	Green alga		Starch production (Miyachi et al. 1986)
NIES-2540	Chlorella variabilis	Green alga	(Paramecium bursaria F36)	Symbiotic (Paramecium bursaria) ; Auxotrophic (Amino acid)

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-267	Calothrix parasitica	Blue-green alga ; Cyanobacteria	Marine (Seaweed)	Endophyte in Nemalion (Rhodophyceae) ; Genome decoded strain
NIES-338	Closterium rostratum	Desmid	Freshwater (Sediment)	Homothallic
NIES-207	Planktothricoides raciborskii	Blue-green alga ; Cyanobacteria	Freshwater (Lake water)	Cyanobacterial water bloom (aoko) ; Planktonic ; Type strain
NIES-39	Arthrospira platensis	Blue-green alga ; Cyanobacteria	Salt water	Autotrophic ; Genome decoded strain (Fujisawa et al. 2010) ; Hydrogen evolution ; Contains good quality of proteins
NIES-408	Achnanthidium minutissimum	Pennate diatom	Freshwater (River water)

Reference
Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 Takamura, N., Kasai, F., Watanabe, M. M. 1988 Differences in the tolerant level of benthic algae to heavy metal - The effects of Cu, Cd, and Zn on the photosynthesis. Res. Rep. Natl. Inst. Environ. Stud., No. 114, 223-232 (in Japanese with English summary). Strain(s): 71, 305, 329, 411, 412, 413, 434, 504, 531, 538, 641, 642, 662 Takamura, N., Kasai, F., Watanabe, M. M. 1989 Effects of Cu, Cd and Zn on photosynthesis of freshwater benthic algae. J. Appl. Phycol., 1, 39-52. Keywords: heavy metal; photosynthesis; algae; tolerance Strain(s): 71, 305, 329, 407, 408, 409, 410, 411, 412, 413, 414, 416, 433, 434, 454, 461, 465, 466, 467, 479, 481, 487, 503, 504, 505, 506, 507, 509, 510, 515, 529, 530, 531, 532, 536, 537, 538, 539, 540, 620, 639, 640, 641, 642, 659, 660, 661, 662 Kimura, J. 2011 Screening for allelopathic activity on algal growth. Bull. Hiroshima Pref. Tech. Res. Inst., Publ. Health Environ. Center, 19, 37-46 (in Japanese). Keywords: Microcystis; Chlorella; Nitzschia; growth inhibition; allelopathy Strain(s): 88, 487, 642 Ng, F.-L., Phang, S.-M., Iwamoto, M., Manaka, T., Thong, C.-H., Shimosawa, K., Periasamy, V., Kumar, G. G., Yunus, K., Fisher, A. C. 2020 Algal biophotovoltaic devices: Surface potential studies. ACS Sustain. Chem. Eng., 8, 10511-10520. Keywords: Algal biophotovoltaic device; Bioelectricity; Biotechnology; Surface potential; Algal biofilm Strain(s): 642 DOI: 10.1021/acssuschemeng.0c02831 Jo, B.-H., Lee, C. S., Song, H.-R., Lee, H.-G., Oh, H.-M. 2014 Development of novel microsatellite markers for strain-specific identification of Chlorella vulgaris. J. Microbiol. Biotechnol., 24, 10.3354/ame01939 Keywords: Chlorella vulgaris; chloroplast; microsatellite; marker; polymorphism; sequence repeat Strain(s): 641, 642, 686, 1269, 2170 PubMed: 24931503 DOI: 10.4014/jmb.1405.05047 Boivin, S., Nagaoka, H., Fujioka, T. 2025 Real-time detection of 3–8-µm microbial particles in sand filter effluent as a surrogate indicator of Cryptosporidium parvum oocysts. Water Res. X, 27, 100345 (article ID). Keywords: Rapid sand filter; Water treatment; Integrity monitoring; Protozoa; Water quality management Strain(s): 642 DOI: 10.1016/j.wroa.2025.100345

Reference

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729

Takamura, N., Kasai, F., Watanabe, M. M. 1988 Differences in the tolerant level of benthic algae to heavy metal - The effects of Cu, Cd, and Zn on the photosynthesis. Res. Rep. Natl. Inst. Environ. Stud., No. 114, 223-232 (in Japanese with English summary).
Strain(s): 71, 305, 329, 411, 412, 413, 434, 504, 531, 538, 641, 642, 662

Takamura, N., Kasai, F., Watanabe, M. M. 1989 Effects of Cu, Cd and Zn on photosynthesis of freshwater benthic algae. J. Appl. Phycol., 1, 39-52.
Keywords: heavy metal; photosynthesis; algae; tolerance
Strain(s): 71, 305, 329, 407, 408, 409, 410, 411, 412, 413, 414, 416, 433, 434, 454, 461, 465, 466, 467, 479, 481, 487, 503, 504, 505, 506, 507, 509, 510, 515, 529, 530, 531, 532, 536, 537, 538, 539, 540, 620, 639, 640, 641, 642, 659, 660, 661, 662

Kimura, J. 2011 Screening for allelopathic activity on algal growth. Bull. Hiroshima Pref. Tech. Res. Inst., Publ. Health Environ. Center, 19, 37-46 (in Japanese).
Keywords: Microcystis; Chlorella; Nitzschia; growth inhibition; allelopathy
Strain(s): 88, 487, 642

Ng, F.-L., Phang, S.-M., Iwamoto, M., Manaka, T., Thong, C.-H., Shimosawa, K., Periasamy, V., Kumar, G. G., Yunus, K., Fisher, A. C. 2020 Algal biophotovoltaic devices: Surface potential studies. ACS Sustain. Chem. Eng., 8, 10511-10520.
Keywords: Algal biophotovoltaic device; Bioelectricity; Biotechnology; Surface potential; Algal biofilm
Strain(s): 642
DOI: 10.1021/acssuschemeng.0c02831

Jo, B.-H., Lee, C. S., Song, H.-R., Lee, H.-G., Oh, H.-M. 2014 Development of novel microsatellite markers for strain-specific identification of Chlorella vulgaris. J. Microbiol. Biotechnol., 24, 10.3354/ame01939
Keywords: Chlorella vulgaris; chloroplast; microsatellite; marker; polymorphism; sequence repeat
Strain(s): 641, 642, 686, 1269, 2170
PubMed: 24931503
DOI: 10.4014/jmb.1405.05047

Boivin, S., Nagaoka, H., Fujioka, T. 2025 Real-time detection of 3–8-µm microbial particles in sand filter effluent as a surrogate indicator of Cryptosporidium parvum oocysts. Water Res. X, 27, 100345 (article ID).
Keywords: Rapid sand filter; Water treatment; Integrity monitoring; Protozoa; Water quality management
Strain(s): 642
DOI: 10.1016/j.wroa.2025.100345

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