Strain Data

Strain number		NIES-42
Phylum		Cyanophyta
Class		Cyanophyceae
Scientific name		Aphanothece elabens (Brébisson ex Meneghini) Elenkin
Synonym
Former name		Microcystis elabens
Common name		Blue-green alga ; Cyanobacteria
Locality (Date of collection)		Lake Kasumigaura, Ibaraki, Japan (1974-08-**)
Latitude / Longitude		36.0428477398 / 140.373233439
Habitat (Isolation source)		Freshwater (Lake water)
History		< IAM (1983);
Isolator (Date of isolation)		Watanabe, Makoto M. (1974-08-**)
Identified by		Otsuka, Shigeto
State of strain		Cryopreservation; Unialgal; Clonal; Axenic
Culture condition (Preculture condition)		Medium: CT Temperature: 25 C Light intensity: 55-70 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M
Gene information		16S rRNA ( AB001724 )
Cell size (min - max)
Organization
Characteristics		Cyanobacterial water bloom (aoko)
Other strain no.		Other collection strain no. : IAM M-177
Remarks		Cryopreserved; Axenic
Movie

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-100	Microcystis aeruginosa	Blue-green alga ; Cyanobacteria	Freshwater (Lake water)	Cyanobacterial water bloom (aoko) ; ST8 (Tanabe et al. 2007)
NIES-2087	Prochlorococcus marinus	Blue-green alga ; Cyanobacteria ; Picoplankton	Marine (Seawater)	Picoplankton ; Genome decoded strain
NIES-89	Microcystis aeruginosa	Blue-green alga ; Cyanobacteria	Freshwater (Lake water)	Cyanobacterial water bloom (aoko) ; Toxicity ; microcystin (+) (Tanabe et al. 2009b) ; ST4 (Tanabe et al. 2007)
NIES-593	Hydrococcus rivularis	Blue-green alga ; Cyanobacteria	Hot spring (Water)	Benthic ; Genome decoded strain (Zhu et al. 2017)
NIES-111	Microcystis aeruginosa	Blue-green alga ; Cyanobacteria	Freshwater (Lake water)	Cyanobacterial water bloom (aoko) ; ST14 (Tanabe et al. 2007)

Reference
Metcalf, J. S., Bell, S. G., Codd, G. A. 2000 Production of novel polyclonal antibodies against the cyanobacterial toxin microcystin-LR and their application for the detection and quantification of microcystins and nodularin. Water Res., 34, 2761-2769. Keywords: cyanobacteria; blue-green algae; microcystin; toxins; antibodies; immunoassays Strain(s): 42, 102, 103, 104, 595 DOI: 10.1016/S0043-1354(99)00429-7 Pan, H., Song, L., Liu, Y., Börner, T. 2002 Detection of hepatotoxic Microcystis strains by PCR with intact cells from both culture and environmental samples. Arch. Microbiol., 178, 421-427. Keywords: Cyanobacteria; Microcystis; Microcystin; Toxin; Peptide synthetase; Whole-cell PCR Strain(s): 42, 90, 98, 101, 595, 610 PubMed: 12420161 DOI: 10.1007/s00203-002-0464-9 Ward, C. J., Beattie, K. A., Lee, E. Y. C., Codd, G. A. 1997 Colorimetric protein phosphatase inhibition assay of laboratory strains and natural blooms of cyanobacteria: comparisons with high-performance liquid chromatographic analysis for microcystins. FEMS Mircobiol. Lett., 153, 465-473. Keywords: Microcystin; Protein phosphatase; Cyanobacteria; High-performance liquid chromatography Strain(s): 42, 102, 103, 104, 298, 595 PubMed: 9271876 DOI: 10.1111/j.1574-6968.1997.tb12611.x Curren, E., Teruaki, Y., Kuwahara, V. S., Leong, S. C. Y. 2019 Rapid profiling of tropical marine cyanobacterial communities. Reg. Stud. Mar. Sci., 25, 100485 (article ID). Keywords: Cyanobacteria; MinION-Oxford Nanopore sequencer; Biodiversity; 16S rRNA amplicon sequencing; Long-read sequencing; Coastal waters; Southeast Asia; Monitoring Strain(s): 22, 25, 42, 843, 2135, 2481 DOI: 10.1016/j.rsma.2018.100485 Asukabe, H., Akahori, S., Ueno, E., Nakayama, T., Yamashita, R., Arii, S., Harada, K., Imanishi, S. Y. 2020 Cyanobacterial classification with the toxicity using MALDI Biotyper. J. Am. Soc. Mass Spectrom., 31, 1572-1578. Keywords: cyanobacteria; MALDI Biotyper; microcystins; profiling Strain(s): 27, 39, 42, 73, 81, 87, 89, 100, 101, 102, 103, 104, 105, 107, 111, 112, 207, 208, 230, 266, 298, 416, 433, 478, 510, 512, 515, 592, 593, 604, 611, 808, 809, 835, 843, 846, 847, 905, 928, 931, 932, 945, 981, 989, 1025, 1043, 1058, 1061, 1064, 1068, 1069, 1071, 1076, 1085, 1099, 1101, 1105, 1113, 1115, 1126, 1133, 1134, 1135, 1144, 1157, 1215, 1642, 1643, 1652, 1668, 1672, 1673, 1677, 1678, 1698, 2087, 2131, 2412, 2665, 3708, 3733, 3734, 3736, 3755, 3756, 3757 PubMed: 32501712 DOI: 10.1021/jasms.0c00148

Reference

Metcalf, J. S., Bell, S. G., Codd, G. A. 2000 Production of novel polyclonal antibodies against the cyanobacterial toxin microcystin-LR and their application for the detection and quantification of microcystins and nodularin. Water Res., 34, 2761-2769.
Keywords: cyanobacteria; blue-green algae; microcystin; toxins; antibodies; immunoassays
Strain(s): 42, 102, 103, 104, 595
DOI: 10.1016/S0043-1354(99)00429-7

Pan, H., Song, L., Liu, Y., Börner, T. 2002 Detection of hepatotoxic Microcystis strains by PCR with intact cells from both culture and environmental samples. Arch. Microbiol., 178, 421-427.
Keywords: Cyanobacteria; Microcystis; Microcystin; Toxin; Peptide synthetase; Whole-cell PCR
Strain(s): 42, 90, 98, 101, 595, 610
PubMed: 12420161
DOI: 10.1007/s00203-002-0464-9

Ward, C. J., Beattie, K. A., Lee, E. Y. C., Codd, G. A. 1997 Colorimetric protein phosphatase inhibition assay of laboratory strains and natural blooms of cyanobacteria: comparisons with high-performance liquid chromatographic analysis for microcystins. FEMS Mircobiol. Lett., 153, 465-473.
Keywords: Microcystin; Protein phosphatase; Cyanobacteria; High-performance liquid chromatography
Strain(s): 42, 102, 103, 104, 298, 595
PubMed: 9271876
DOI: 10.1111/j.1574-6968.1997.tb12611.x

Curren, E., Teruaki, Y., Kuwahara, V. S., Leong, S. C. Y. 2019 Rapid profiling of tropical marine cyanobacterial communities. Reg. Stud. Mar. Sci., 25, 100485 (article ID).
Keywords: Cyanobacteria; MinION-Oxford Nanopore sequencer; Biodiversity; 16S rRNA amplicon sequencing; Long-read sequencing; Coastal waters; Southeast Asia; Monitoring
Strain(s): 22, 25, 42, 843, 2135, 2481
DOI: 10.1016/j.rsma.2018.100485

Asukabe, H., Akahori, S., Ueno, E., Nakayama, T., Yamashita, R., Arii, S., Harada, K., Imanishi, S. Y. 2020 Cyanobacterial classification with the toxicity using MALDI Biotyper. J. Am. Soc. Mass Spectrom., 31, 1572-1578.
Keywords: cyanobacteria; MALDI Biotyper; microcystins; profiling
Strain(s): 27, 39, 42, 73, 81, 87, 89, 100, 101, 102, 103, 104, 105, 107, 111, 112, 207, 208, 230, 266, 298, 416, 433, 478, 510, 512, 515, 592, 593, 604, 611, 808, 809, 835, 843, 846, 847, 905, 928, 931, 932, 945, 981, 989, 1025, 1043, 1058, 1061, 1064, 1068, 1069, 1071, 1076, 1085, 1099, 1101, 1105, 1113, 1115, 1126, 1133, 1134, 1135, 1144, 1157, 1215, 1642, 1643, 1652, 1668, 1672, 1673, 1677, 1678, 1698, 2087, 2131, 2412, 2665, 3708, 3733, 3734, 3736, 3755, 3756, 3757
PubMed: 32501712
DOI: 10.1021/jasms.0c00148

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