A big image opens when the thumbnail is clicked.(nies-2330.jpg)
Strain number NIES-2330  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Didymogenes soliella Hoshina & Fujiwara  
Synonym  
Former name Chlorella sp.  
Common name Green alga  
Locality (Date of collection) Lake Kasumigaura, Miho, Ibaraki, Japan (1991-04-22)  
Latitude / Longitude 36.0428477398 / 140.373233439 
Habitat (Isolation source) Freshwater (Pond sediment)  
History < Watanabe, Yoshitomo  
Isolator (Date of isolation) Watanabe, Yoshitomo (1991-06-**)  
Identified by Hoshina, Ryo (Reidentify)  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Mar]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 (25) C
Light intensity:  4-10 (14-16) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M (14 D)  
Gene information 18S-ITS1-5.8S-ITS2-28S ( AB731605 )  
Cell size (min - max) 2 - 7 μm  
Organization Unicellular 
Characteristics Authentic strain (Hoshina et al. 2013)  
Other strain no. Other strain no. : HA-1  
Remarks Cryopreserved; Axenic 
Movie  
Related strain information
(Large number of orders and same genus or species)
Related strain information
(Used in same reference)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-2171
Micractinium inermum Green alga   Authentic strain (Hoshina et al. 2013)  
NIES-455
Micractinium bornhemiensis Green alga   Freshwater (Pond sediment)  
NIES-4034
Carolibrandtia ciliaticola Green alga   Freshwater (Stokesia vernalis) Symbiotic  
NIES-2173
Chlorella sorokiniana Green alga   Starch production (Miyachi et al. 1986)  
NIES-4032
Carolibrandtia ciliaticola Green alga   Freshwater (Cyclotrichium viride) Symbiotic  
Reference
Hoshina, R. & Fujiwara, Y. 2013 Molecular characterization of Chlorella cultures of the National Institute for Environmental Studies culture collection with description of Micractinium inermum sp. nov., Didymogenes sphaerica sp. nov., Didymogenes soliella sp. nov., (Chlorellaceae, Trebouxiophyceae). Phycol. Res., 61, 124-132.
Keywords: Chlorella; Didymogenes; group 1 intron; internal transcribed spacer 2; Micractinium; species concept
Strain(s): 2167217121732330 
DOI: 10.1111/pre.12010

Krivina, E., Portnov, A., Temraleeva, A. 2024 A description of Aliichlorella ignota gen. et sp. nov. and a comparison of the efficiency of species delimitation methods in the Chlorella-clade (Trebouxiophyceae, Chlorophyta). Phycol. Res., 72, 180-190.
Keywords: CBC approach; delimitation algorithms; green microalgae, morphology; taxonomy
Strain(s): 4552167217121732330403240334034 
DOI: 10.1111/pre.12551

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