Strain Data

Strain number		NIES-686
Phylum		Chlorophyta
Class		Trebouxiophyceae
Scientific name		Chlorella vulgaris Beijerinck var. vulgaris
Synonym
Former name
Common name		Green alga
Locality (Date of collection)		Delft, Netherland
Latitude / Longitude
Habitat (Isolation source)
History		< Katagiri, Masayuki < CCAP
Isolator (Date of isolation)		Beijerinck, M. W. (1892--)
Identified by		Confirmed by DNA sequencing at NIES
State of strain		Cryopreservation; Unialgal; Clonal; Axenic[2021 Jul]
Culture condition (Preculture condition)		Medium: C (agar) Temperature: 20 (25) C Light intensity: 4-10 (14-16) µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 3 M (21 D)
Gene information		18S rRNA ( AB488578 )
Cell size (min - max)		- 3 μm
Organization		Unicellular
Characteristics		Authentic strain
Other strain no.		Other collection strain no. : CCAP 211/11b; IAM C-207; SAG 211-11b Other strain no. : C-207
Remarks		Cryopreserved; Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-2170	Chlorella vulgaris	Green alga		Starch production (Hirokawa et al. 1982) ; Genome decoded strain (Wakasugi et al. 1997)
NIES-1269	Chlorella vulgaris	Green alga	Marine (Tidal flat water)
NIES-641	Chlorella vulgaris	Green alga	Freshwater (River water)
NIES-642	Chlorella vulgaris	Green alga	Freshwater (River water)
NIES-227	Chlorella vulgaris	Green alga

Related strain information (Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-2169	Chlorella sorokiniana	Green alga	Freshwater	Starch production (Takeshita et al. 2014)
NIES-2173	Chlorella sorokiniana	Green alga		Starch production (Miyachi et al. 1986)
NIES-2540	Chlorella variabilis	Green alga	(Paramecium bursaria F36)	Symbiotic (Paramecium bursaria) ; Auxotrophic (Amino acid)

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-541	Volvox aureus	Green alga ; Volvocine alga	Freshwater (Sediment)	Phototaxis
NIES-536	Ulothrix zonata		Freshwater (Water)
NIES-2352	Chloroidium saccharophilum	Green alga
NIES-361	Oscillatoria amphibia	Blue-green alga ; Cyanobacteria	Marine (Seawater)	Benthic
NIES-139	Gonatozygon brebissonii	Desmid	Freshwater

Reference
Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 Nozaki, H., Katagiri, M., Nakagawa, M., Aizawa, K., Watanabe, M. M. 1995 Taxonomic re-examination of the two strains labeled Chlorella in the Microbial Culture Collection at the National Institute for Environmental Studies (NIES-Collection). Microbiol. Cult. Coll., 11, 11-18. Keywords: Chlorella; Graesiella emersonii comb. nov.; green algae; morphology; taxonomy Strain(s): 226, 227, 686, 687, 688, 689, 690 Yamada, T. & Sakaguchi, K. 1982 Comparative studies on Chlorella cell walls: Induction of protoplast formation. Arch. Mikrobiol., 132, 10-13. Strain(s): 227, 686, 2159, 2163, 2166, 2168, 2171, 2189, 2352 DOI: 10.1007/BF00690809 Cannell, R. J. P., Owsianka, A. M., Walker, J. M. 1988 Results of a large-scale screening programme to detect antibacterial activity from freshwater algae. Br. Phycol. J., 23, 41-44. Strain(s): 686, 2287 DOI: 10.1080/00071618800650051 Kessler, E. & Huss, V. A. R. 1992 Comparative physiology and biochemistry and taxonomic assignment of the Chlorella (Chlorophyceae) strains of the Culture Collection of the University of Texas at Austin. J. Phycol., 28, 550-553. Keywords: chemotaxonomy; Chlorella; Chlorophyta; UTEX culture collection; utilization for research and biotechnology Strain(s): 686, 687, 2150, 2151, 2160, 2163, 2169, 2175, 2176 DOI: 10.1111/j.0022-3646.1992.00550.x Suda, K., Tanji, K., Hori, K., Unno, H. 1999 Evidence for a novel Chlorella virus -encoded alginate lyase. FEMS Microbiol. Lett., 180, 45-53. Keywords: Chlorella; virus; alginate lyase; lysis Strain(s): 686, 843, 2170 PubMed: 10547443 DOI: 10.1111/j.1574-6968.1999.tb08776.x Jo, B.-H., Lee, C. S., Song, H.-R., Lee, H.-G., Oh, H.-M. 2014 Development of novel microsatellite markers for strain-specific identification of Chlorella vulgaris. J. Microbiol. Biotechnol., 24, 10.3354/ame01939 Keywords: Chlorella vulgaris; chloroplast; microsatellite; marker; polymorphism; sequence repeat Strain(s): 641, 642, 686, 1269, 2170 PubMed: 24931503 DOI: 10.4014/jmb.1405.05047 Morimoto. M., Toyoshima, H., Shimamura, M., Matsuzaki, R., Takeshita, S., Kawasaki, S. 2026 Apricichlorella intertidalis gen. et sp. nov. and Chlorella intertidalis sp. nov. (Chlorellaceae, Chlorellales), isolated from rock surfaces of the intertidal zone in Japan. Phycol. Res., 74, 81-93. Keywords: cell wall thickening; chlorella; dehydration stress; intertidal zone; oil accumulation; photooxidative stress; salt stress Strain(s): 686, 4672, 4673 DOI: 10.1111/pre.70022

Reference

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729

Nozaki, H., Katagiri, M., Nakagawa, M., Aizawa, K., Watanabe, M. M. 1995 Taxonomic re-examination of the two strains labeled Chlorella in the Microbial Culture Collection at the National Institute for Environmental Studies (NIES-Collection). Microbiol. Cult. Coll., 11, 11-18.
Keywords: Chlorella; Graesiella emersonii comb. nov.; green algae; morphology; taxonomy
Strain(s): 226, 227, 686, 687, 688, 689, 690

Yamada, T. & Sakaguchi, K. 1982 Comparative studies on Chlorella cell walls: Induction of protoplast formation. Arch. Mikrobiol., 132, 10-13.
Strain(s): 227, 686, 2159, 2163, 2166, 2168, 2171, 2189, 2352
DOI: 10.1007/BF00690809

Cannell, R. J. P., Owsianka, A. M., Walker, J. M. 1988 Results of a large-scale screening programme to detect antibacterial activity from freshwater algae. Br. Phycol. J., 23, 41-44.
Strain(s): 686, 2287
DOI: 10.1080/00071618800650051

Kessler, E. & Huss, V. A. R. 1992 Comparative physiology and biochemistry and taxonomic assignment of the Chlorella (Chlorophyceae) strains of the Culture Collection of the University of Texas at Austin. J. Phycol., 28, 550-553.
Keywords: chemotaxonomy; Chlorella; Chlorophyta; UTEX culture collection; utilization for research and biotechnology
Strain(s): 686, 687, 2150, 2151, 2160, 2163, 2169, 2175, 2176
DOI: 10.1111/j.0022-3646.1992.00550.x

Suda, K., Tanji, K., Hori, K., Unno, H. 1999 Evidence for a novel Chlorella virus -encoded alginate lyase. FEMS Microbiol. Lett., 180, 45-53.
Keywords: Chlorella; virus; alginate lyase; lysis
Strain(s): 686, 843, 2170
PubMed: 10547443
DOI: 10.1111/j.1574-6968.1999.tb08776.x

Jo, B.-H., Lee, C. S., Song, H.-R., Lee, H.-G., Oh, H.-M. 2014 Development of novel microsatellite markers for strain-specific identification of Chlorella vulgaris. J. Microbiol. Biotechnol., 24, 10.3354/ame01939
Keywords: Chlorella vulgaris; chloroplast; microsatellite; marker; polymorphism; sequence repeat
Strain(s): 641, 642, 686, 1269, 2170
PubMed: 24931503
DOI: 10.4014/jmb.1405.05047

Morimoto. M., Toyoshima, H., Shimamura, M., Matsuzaki, R., Takeshita, S., Kawasaki, S. 2026 Apricichlorella intertidalis gen. et sp. nov. and Chlorella intertidalis sp. nov. (Chlorellaceae, Chlorellales), isolated from rock surfaces of the intertidal zone in Japan. Phycol. Res., 74, 81-93.
Keywords: cell wall thickening; chlorella; dehydration stress; intertidal zone; oil accumulation; photooxidative stress; salt stress
Strain(s): 686, 4672, 4673
DOI: 10.1111/pre.70022

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