Strain number NIES-2267  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Monoraphidium sp.  
Synonym  
Former name  
Common name Green alga  
Locality (Date of collection) Yamaguchi, Japan  
Latitude / Longitude  
Habitat (Isolation source) (Activated sludge)  
History < IAM (2007) < Fujii, Katsuhiko  
Isolator (Date of isolation) Fujii, Katsuhiko  
Identified by Fujii, Katsuhiko  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Mar]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  3-5 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  4 M  
Gene information  
Cell size (min - max) - 200 μm  
Organization Unicellular 
Characteristics Astaxanthin production  
Other strain no. Other collection strain no. : IAM C-632
Other strain no. : GK 12  
Remarks Cryopreserved; Axenic 
Movie  
Related strain information
(Large number of orders and same genus or species)
Reference
Fujii, K., Nakashima, H., Hashidzume, Y., Uchiyama, T., Mishiro, K., Kadota, Y. 2010 Potential use of the astaxanthin-producing microalga, Monoraphidium sp. GK12, as a functional aquafeed for prawns. J. Appl. Phycol., 22, 363-369.
Keywords: Astaxanthin; Vitamin; Unsaturated fatty acid; Microalgae; Monoraphidium; Functional aquafeed
Strain(s): 2267 
DOI: 10.1007/s10811-009-9468-z

Fujii, K. 2012 Process integration of supercritical carbon dioxide extraction and acid treatment for astaxanthin extraction from a vegetative microalga. Food Bioprod. Process., 90, 762-766.
Keywords: Supercritical carbon dioxide; Microalgae; Monoraphidium sp.; Astaxanthin; Chlorophyll
Strain(s): 2267 
DOI: 10.1016/j.fbp.2012.01.006

Fujii, K., Nakajima, H, Anno, Y. 2008 Potential of Monoraphidium sp. GK12 for energy-saving astazanthin production. J. Chem. Tech. Biotechnol., 83, 1578-1584.
Keywords: astaxanthin; microalgae; Monoraphidium sp.; outdoor cultivation; energy-saving technology
Strain(s): 2267 
DOI: 10.1002/jctb.1978

Fujii, K., Imazato, E., Nakashima, H., Ooi, O., Saeki, A. 2006 Isolation of the non-fastidious microalga with astaxanthin-accumulating property and its potential for application to aquaculture. Aquaculture, 261, 285-293.
Keywords: Astaxanthin; Aquaculture; Microalgae; Monoraphidium
Strain(s): 2267 
DOI: 10.1016/j.aquaculture.2006.07.014

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