Strain number NIES-2564  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Pyrobotrys squarrosa (Korshikov) Korshikov  
Synonym Chlamydobotrys squarrosa Korshikov 1928; Uva squarrosa (Korshikov) Fott 1967; Pyrobotrys squarrosa (Korshikov) Huber-Pestalozzi in Fott 1967  
Former name  
Common name Green alga  
Locality (Date of collection) Otsuka-minami, Toyama, Toyama, Japan (2005-09-23)  
Latitude / Longitude 36.72722222 / 137.16333333 
Habitat (Isolation source) Freshwater (Paddy soil)  
History < Nakada, Takashi  
Isolator (Date of isolation) Nakada, Takashi (2009-07-16)  
Identified by Nakada, Takashi  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  AFAC  
Temperature:  20 C
Light intensity:  7-13 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S rRNA ( AB542919 ) , atpB ( LC066350 ) , psaB ( AB542924 ) , rbcL ( AB542927 )  
Cell size (min - max) 8 - 18 μm  
Organization  
Characteristics Mixotrophic ; Osmotrophic ; Resting spore-forming  
Other strain no. Other collection strain no. : NBRC 107039
Other strain no. : KrCl905  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic 
Movie  
Related strain information
(Large number of orders and same genus or species)
Related strain information
(Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-3771
Pyrobotrys elongata Green alga   Freshwater (Paddy soil) Mixotrophic ; Osmotrophic ; Phototaxis ; Microaerophilic  
NIES-3772
Pyrobotrys elongata Green alga   Freshwater (Paddy soil) Mixotrophic ; Osmotrophic ; Phototaxis ; Microaerophilic  
NIES-3773
Pyrobotrys squarrosa Green alga   Freshwater (Sediment) Mixotrophic ; Osmotrophic ; Phototaxis ; Microaerophilic  
NIES-3774
Pyrobotrys squarrosa Green alga   Freshwater (Sediment) Mixotrophic ; Osmotrophic ; Phototaxis ; Microaerophilic  
Related strain information
(Used in same reference)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-569
Gonium pectorale Green alga   Freshwater (Paddy soil) Phototaxis ; Heterothallic ; Isogamous ; Mating type (+) ; Crosses with NIES-570  
NIES-432
Carteria radiosa Green alga   Freshwater (Pond water)  
NIES-1048
Chlamydomonas noctigama Green alga   Freshwater Phototaxis  
NIES-2293
Hamakko caudatus Green alga   Freshwater (Soil) Authentic strain (Nakada, T. et al. 2009)  
NIES-421
Carteria crucifera Green alga   Freshwater (Sediment)  
Reference
Nakada, T., Nozaki, H., Tomita, M. 2010 Another origin of coloniality in volvocaleans: the phylogenetic position of Pyrobotrys Arnoldi (Spondylomoraceae, Volvocales). J. Eucaryot. Microbiol., 57, 379-382.
Keywords: Chlorophyceae; molecular phylogeny; multicellularity
Strain(s): 2564 
PubMed: 20553352
DOI: 10.1111/j.1550-7408.2010.00488.x

Munakata, H., Nakada, T., Nakahigashi, K., Nozaki, H., Tomita, M. 2016 Phylogenetic position and molecular chronology of a colonial green flagellate, Stephanosphaera pluvialis (Volvocales, Chlorophyceae), among unicellular algae. J. Eucaryot. Microbiol., 63, 340-348.
Keywords: Balticola; coloniality; divergence time; evolution; molecular phylogeny
Strain(s): 12314442142843243944656756957165686188796810482293256441244125 
PubMed: 26595722
DOI: 10.1111/jeu.12283

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