Strain Data

Strain number		NIES-641
Phylum		Chlorophyta
Class		Trebouxiophyceae
Scientific name		Chlorella vulgaris Beijerinck var. vulgaris
Synonym
Former name
Common name		Green alga
Locality (Date of collection)		Miyata River, Ibaraki, Japan (1987-02-25)
Latitude / Longitude
Habitat (Isolation source)		Freshwater (River water)
History		< Kasai, Fumie
Isolator (Date of isolation)		Kasai, Fumie (1987-03-23)
Identified by		Kasai, Fumie; Confirmed by DNA sequencing at NIES
State of strain		Cryopreservation; Unialgal; Clonal; Axenic[2018 Mar]
Culture condition (Preculture condition)		Medium: C Temperature: 20 C Light intensity: 8-15 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 6 M
Gene information		18S rRNA ( AB488576 )
Cell size (min - max)		2 - 5 μm
Organization		Unicellular
Characteristics
Other strain no.		Other strain no. : 1st-3-26
Remarks		Cryopreserved; Axenic
Movie

Related strain information (Large number of orders and same genus or species)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-2170	Chlorella vulgaris	Green alga		Starch production (Hirokawa et al. 1982) ; Genome decoded strain (Wakasugi et al. 1997)
NIES-1269	Chlorella vulgaris	Green alga	Marine (Tidal flat water)
NIES-642	Chlorella vulgaris	Green alga	Freshwater (River water)
NIES-686	Chlorella vulgaris	Green alga		Authentic strain
NIES-227	Chlorella vulgaris	Green alga

Related strain information (Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-2169	Chlorella sorokiniana	Green alga	Freshwater	Starch production (Takeshita et al. 2014)
NIES-2173	Chlorella sorokiniana	Green alga		Starch production (Miyachi et al. 1986)
NIES-2540	Chlorella variabilis	Green alga	(Paramecium bursaria F36)	Symbiotic (Paramecium bursaria) ; Auxotrophic (Amino acid)

Related strain information (Used in same reference)
Strain number	Scientific name	Common name	Habitat (Isolation source)	Characteristics
NIES-583	Volvulina compacta	Green alga	Freshwater (Paddy soil)	Phototaxis ; Heterothallic ; Mating type (−) ; Crosses with NIES-582
NIES-512	Pseudanabaena foetida	Blue-green alga ; Cyanobacteria	Freshwater (Pond water)	Offensive odor ; Test strain (Nagai, T., 2016) ; 2-MIB production (Iwase et al. 2010) ; Type strain (Tuji & Niiyama 2016)
NIES-380	Eremosphaera viridis	Green alga	Freshwater (Pond water)
NIES-458	Eudorina elegans	Green alga	Freshwater (Paddy soil)	Phototaxis ; Homothallic ; Monoecious
NIES-33	Oscillatoria tenuis	Blue-green alga ; Cyanobacteria

Reference
Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 Takamura, N., Kasai, F., Watanabe, M. M. 1988 Differences in the tolerant level of benthic algae to heavy metal - The effects of Cu, Cd, and Zn on the photosynthesis. Res. Rep. Natl. Inst. Environ. Stud., No. 114, 223-232 (in Japanese with English summary). Strain(s): 71, 305, 329, 411, 412, 413, 434, 504, 531, 538, 641, 642, 662 Takamura, N., Kasai, F., Watanabe, M. M. 1989 Effects of Cu, Cd and Zn on photosynthesis of freshwater benthic algae. J. Appl. Phycol., 1, 39-52. Keywords: heavy metal; photosynthesis; algae; tolerance Strain(s): 71, 305, 329, 407, 408, 409, 410, 411, 412, 413, 414, 416, 433, 434, 454, 461, 465, 466, 467, 479, 481, 487, 503, 504, 505, 506, 507, 509, 510, 515, 529, 530, 531, 532, 536, 537, 538, 539, 540, 620, 639, 640, 641, 642, 659, 660, 661, 662 Lu, J., Takeuchi, T., Satoh, H. 2004 Ingestion and assimilation of three species of freshwater algae by larval tilapia Oreochromis niloticus. Aquaculture, 238, 437-449. Keywords: carbon assimilation; closed ecological recirculating aquaculture system (CERAS); Chlorella; 14C labeling; Euglena; ingestion; Oreochromis niloticus; Spirulina Strain(s): 39, 48, 641 DOI: 10.1016/j.aquaculture.2004.05.002 Jo, B.-H., Lee, C. S., Song, H.-R., Lee, H.-G., Oh, H.-M. 2014 Development of novel microsatellite markers for strain-specific identification of Chlorella vulgaris. J. Microbiol. Biotechnol., 24, 10.3354/ame01939 Keywords: Chlorella vulgaris; chloroplast; microsatellite; marker; polymorphism; sequence repeat Strain(s): 641, 642, 686, 1269, 2170 PubMed: 24931503 DOI: 10.4014/jmb.1405.05047

Reference

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729

Takamura, N., Kasai, F., Watanabe, M. M. 1988 Differences in the tolerant level of benthic algae to heavy metal - The effects of Cu, Cd, and Zn on the photosynthesis. Res. Rep. Natl. Inst. Environ. Stud., No. 114, 223-232 (in Japanese with English summary).
Strain(s): 71, 305, 329, 411, 412, 413, 434, 504, 531, 538, 641, 642, 662

Takamura, N., Kasai, F., Watanabe, M. M. 1989 Effects of Cu, Cd and Zn on photosynthesis of freshwater benthic algae. J. Appl. Phycol., 1, 39-52.
Keywords: heavy metal; photosynthesis; algae; tolerance
Strain(s): 71, 305, 329, 407, 408, 409, 410, 411, 412, 413, 414, 416, 433, 434, 454, 461, 465, 466, 467, 479, 481, 487, 503, 504, 505, 506, 507, 509, 510, 515, 529, 530, 531, 532, 536, 537, 538, 539, 540, 620, 639, 640, 641, 642, 659, 660, 661, 662

Lu, J., Takeuchi, T., Satoh, H. 2004 Ingestion and assimilation of three species of freshwater algae by larval tilapia Oreochromis niloticus. Aquaculture, 238, 437-449.
Keywords: carbon assimilation; closed ecological recirculating aquaculture system (CERAS); Chlorella; 14C labeling; Euglena; ingestion; Oreochromis niloticus; Spirulina
Strain(s): 39, 48, 641
DOI: 10.1016/j.aquaculture.2004.05.002

Jo, B.-H., Lee, C. S., Song, H.-R., Lee, H.-G., Oh, H.-M. 2014 Development of novel microsatellite markers for strain-specific identification of Chlorella vulgaris. J. Microbiol. Biotechnol., 24, 10.3354/ame01939
Keywords: Chlorella vulgaris; chloroplast; microsatellite; marker; polymorphism; sequence repeat
Strain(s): 641, 642, 686, 1269, 2170
PubMed: 24931503
DOI: 10.4014/jmb.1405.05047

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