Strain Data
Strain number NIES-968  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chlamydomonas kuwadae Gerloff  
Synonym  
Former name  
Common name Green alga  
Locality (Date of collection) Lake Nojiri (Depth 0- -10m), Nagano, Japan (1992-05-13)  
Latitude / Longitude 36.823118 / 138.222841 
Habitat (Isolation source) Freshwater (Lake water)  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (1992-05-14)  
Identified by Nozaki, Hisayoshi  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition) 		Medium:  AF-6  
Temperature:  10 C
Light intensity:  6-8 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 18S rRNA ( AB451190 AB753038 ) , atpB ( AB084318 ) , psaB ( AB084356 ) , rbcL ( AB084334 )  
Cell size (min - max) 10 - 25 μm  
Organization Unicellular; Flagellate 
Characteristics Phototaxis  
Other strain no. Other strain no. : 92-514-H-13  
Remarks Cryopreserved; Axenic 
Movie  
Related strain information
(Large number of orders and same genus or species)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-2235
 		Chlamydomonas reinhardtii Green alga   Phototaxis ; Starch production (Kuchitsu et al. 1988) ; Genome decoded strain ; Mating type (−) ; Crosses with NIES-2239 (IAM C-562) ; Live food (Liu et al. 2016)  
NIES-2236
 		Chlamydomonas reinhardtii Green alga   Terrestrial (Soil) Phototaxis ; Mating type (+)  
NIES-2237
 		Chlamydomonas reinhardtii Green alga   Phototaxis ; D-Threonine aldolase (Hirato et al. 2007) ; D-amino acids ; Cell wall less mutant of NIES-2238 ; Loss of nitrate reductase ; Unable to use nitrate as nitrogen source (Pröschold et al. 2005)  
NIES-2238
 		Chlamydomonas reinhardtii Green alga   Terrestrial (Soil) Phototaxis ; Mating type (+) ; Loss of nitrate reductase ; Unable to use nitrate as nitrogen source (Pröschold et al. 2005)  
NIES-2463
 		Chlamydomonas reinhardtii Green alga   Freshwater (Soil) Phototaxis ; Heterothallic ; Dioecious ; Isogamous ; Mating type (+)  
Related strain information
(Whose relevance due to simultaneous purchases and other information is inferred by AI)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-2499
 		Chlamydomonas eustigma Green alga   Freshwater (Microfilm) Acidophilic ; Phototaxis ; Arsenic tolerance (Hirooka et al. 2017) ; Genome decoded strain (Hirooka et al. 2017)  
NIES-2464
 		Chlamydomonas reinhardtii Green alga   Freshwater (Soil) Phototaxis ; Heterothallic ; Dioecious ; Isogamous ; Mating type (−)  
Related strain information
(Used in same reference)
Strain number Scientific name Common name Habitat (Isolation source) Characteristics
NIES-2248
 		Chlamydomonas zebra Green alga   Freshwater Phototaxis  
NIES-2213
 		Chlamydomonas noctigama Green alga   Phototaxis  
NIES-564
 		Astrephomene perforata Green alga   Freshwater (Paddy soil) Phototaxis ; Authentic strain ; Heterothallic ; Isogamous ; Mating type (+) ; Crosses with NIES-565  
NIES-2317
 		Chlamydomonas sp. Green alga   Freshwater (Paddy soil) Phototaxis ; Mating type (−)  
NIES-861
 		Pteromonas angulosa Green alga   Planktonic  
Reference
Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430.
Keywords: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales
Strain(s): 96,  123,  144,  213,  418,  421,  425,  428,  432,  439,  446,  447,  456,  474,  522,  564,  566,  567,  569,  571,  574,  582,  653,  732,  851,  858,  861,  875,  882,  883,  968 
PubMed: 12355262
DOI: 10.1007/s00239-002-2338-9

Nakada, T. & Nozaki, H. 2009 Taxonomic study of two new genera of fusiform green flagellates, Tabris gen. nov. and Hamakko gen. nov. (Volvocales, Chlorophyceae). J. Phycol., 45, 482-492.
Keywords: Chlorogonium; Hamakko gen. nov.; molecular phylogeny; Tabris gen. nov.; taxonomy; ultrastructure; Volvocales
Strain(s): 122,  637,  748,  752,  760,  968,  1358,  1359,  1849,  1850,  1869,  2293,  2294,  2295,  4125 
PubMed: 27033826
DOI: 10.1111/j.1529-8817.2009.00652.x

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 158,  437,  438,  440,  884,  968,  1021,  1022,  1048,  1733,  1848,  1849,  2201,  2202,  2203,  2204,  2206,  2207,  2208,  2209,  2210,  2211,  2212,  2213,  2214,  2215,  2216,  2218,  2219,  2220,  2222,  2223,  2224,  2225,  2228,  2229,  2230,  2231,  2233,  2234,  2235,  2236,  2237,  2238,  2239,  2240,  2241,  2242,  2243,  2246,  2247,  2248,  2314,  2315,  2316,  2317,  2318,  2319,  2320,  2321,  2322,  2323,  2324,  2462,  2463,  2499,  2575,  2576,  2577,  2578,  2579,  3284 
DOI: 10.60369/microresys.29.1_1

Munakata, H., Nakada, T., Nakahigashi, K., Nozaki, H., Tomita, M. 2016 Phylogenetic position and molecular chronology of a colonial green flagellate, Stephanosphaera pluvialis (Volvocales, Chlorophyceae), among unicellular algae. J. Eucaryot. Microbiol., 63, 340-348.
Keywords: Balticola; coloniality; divergence time; evolution; molecular phylogeny
Strain(s): 123,  144,  421,  428,  432,  439,  446,  567,  569,  571,  656,  861,  887,  968,  1048,  2293,  2564,  4124,  4125 
PubMed: 26595722
DOI: 10.1111/jeu.12283

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